《植物生理学报》 2013, 49(12): 1359-1362

单头亚菊的组织培养与快速繁殖

红歌, 吴潇波, 谢菲, 赵惠恩*

北京林业大学园林学院, 北京100083

通信作者：赵惠恩；E-mail: zhaohuien@bjfu.edu.cn；Tel: 010-62338817

摘　要：

以单头亚菊茎段为外植体对其进行组织培养, MS为基本培养基, 设置不同激素浓度配比。对实验结果进行观察分析, 筛选出合适的配方。启动培养基为MS+0.5 mg·L^-1 6-BA+0.01 mg·L^-1 NAA。继代培养基为MS+0.75 mg·L^-1 6-BA+0.01 mg·L^-1 NAA, 可获得较高的增殖率。不定根最适诱导培养基为1/2MS+0.15 mg·L^-1 IBA, 生根率达87%以上, 组培苗移栽成活率达98%。

关键词：单头亚菊; 组织培养; 快速繁殖

收稿：2013-07-15   修定：2013-10-13

资助：国家自然科学基金(30970207)

Tissue Culture and Rapid Propagation of Ajania scharnhorstii (Rgl. et Schmalh.) Tzvel

HONG Ge, WU Xiao-Bo, XIE Fei, ZHAO Hui-En*

College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Corresponding author: ZHAO Hui-En; E-mail: zhaohuien@bjfu.edu.cn; Tel: 010-62338817

Abstract:

The tissue culture technology of Ajania scharnhorstii was studied in this research. Bud formation on the nodal explants was induced on an optimal medium MS+0.5 mg·L^-1 6-BA+0.01 mg·L^-1 NAA. To get a high propagation ratio, the seedlings were transferred to the medium of MS+0.75 mg·L^-1 6-BA+0.01 mg·L^-1 NAA. The optimal medium for adventitious root inducing was 1/2MS+0.15 mg·L^-1 IBA and the rooting rate was at least 87%. The rooted and acclimated plantlets were transferred outdoors with 98% transplantation success.

Key words: Ajania scharnhorstii; tissue culture; rapid propagation